Bio-Rad Laboratories- NGC Discover 100 Pro Chromatography System

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Features and Benefits of the NGC Discover 100 Pro System

  • Automated 100 ml/min pumps provide accurate gradients for high-resolution separation of biomolecules
  • Automated injection of large sample volumes using fixed or dynamic loops
  • Gradient separations at different pH values and buffer pH monitoring, for rapid method development
  • Automated buffer and salt gradients with buffer blending during automated inline buffer preparation at flow rates of up to 200 ml/min, for rapid scouting of purification conditions
  • Simultaneous four-wavelength detection of proteins, peptides, nucleic acids, and chromophores, with conductivity measurement and pH detection
  • Fraction collection in combination with large-volume collection using a 12-port outlet valve
  • Compatibility with the C-96 Autosampler, available with or without cooling, for accurate and reproducible sample injections
  • Compatibility with the NGC Fraction Collector and BioFrac™ Fraction Collector for sample collection

The NGC Discover 100 Pro System includes the following modules and kits:

  • 1 NGC Two-Tier Base Frame
  • 1 NGC 3rd Tier Expansion Frame
  • 1 NGC 4th Tier Expansion Frame
  • 1 NGC Sample Pump Module
  • 2 NGC F100 Pump Modules for a flow rate of 0.01–100 ml/min at 1,450 psi (100 bar, 10 MPa)
  • 2 NGC Inlet Valve Modules
  • 1 NGC Column Switching Valve for switching between columns and media without the need to replumb
  • 1 NGC Outlet Valve Module for collecting up to 10 fractions larger than 5 ml
  • 1 NGC Mixer Module and 750 µl base and top assembly, includes an extension mixer barrel (5 ml)
  • 1 NGC Multi-Wavelength Detector Module for simultaneous four-wavelength detection at 190–800 nm and salt gradient monitoring with integrated conductivity monitor, includes tubing and fittings and one 5 mm flow cell
  • 1 NGC Sample Inject Valve Module for automated application of small sample volumes from sample loops or large sample volumes using an optional sample pump
  • 1 NGC Buffer Blending Valve Module
  • 1 NGC pH Valve Module, includes 1 NGC pH Probe (Ag/AgCl)
  • 1 integrated system touch screen
  • 1 copy of ChromLab™ Software for instrument control, data collection, and data analysis
  • 1 documentation DVD containing manuals for use with NGC Systems
  • 1 NGC Buffer Tray
  • 1 NGC Communication Adaptor for connecting the BioFrac Fraction C