4-Well Cassette Options and DetailsThree cassette designs provide a variety of process conditions.
- Standard [REG]
- Baffled [BFL]
- Central Vent [PRC]
Polycarbonate, irradiated by e-beam
Three Options for Well Closures
?Type A: Single-use with neoprene check valve and sterile membrane barrier
Type D: Single-use with sterile membrane barrier
Type E: Autoclavable, re-usable, with stainless steel check valve, no sterile membrane barrier
Recommended cassette/closure combination for specific applications
Culture Type
Bacteria
Cell Density All OD600 < 40OD 600 > 40
O2 Conditions Anaerobic Aerobic
pH Conditions 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0
Cassette PRCLOW / PRC PRCLOW / PRC REGLOW / REG
Cap TypeA/E TypeA/E TypeD
Gas1 CO2 (optional)
Gas2 NH3/N2 NH3/Air
Gas3 N2 O2
Yeast
Cell Density All OD600 < 30OD 600 > 30
O2 Conditions Anaerobic Aerobic
pH Conditions 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0 - 8.0
Cassette PRCLOW / PRC PRCLOW / PRC REGLOW / REG
Cap TypeA/E TypeA/E TypeD
Gas1
Gas2 NH3/N2 NH3/Air
Gas3 N2 O2
Culture Type
Filamentous Fungi
Cell Density All
O2 Conditions Anaerobic Aerobic
pH Conditions 3.5 - 5.5 / 6.0 - 8.0 3.5 - 5.5 / 6.0
Cassette BFLLOW / BFL BFLLOW / BFL
Cap TypeA/E TypeD
Gas1 CO2 (optional)
Gas2 NH3/N2 NH3/Air
Gas3 N2 O2
Cell Culture
Cell Density All
O2 Conditions < 50% DO > 50% DO
pH Conditions 6.0 - 8.0
Cassette PRC
Cap TypeA/E TypeD
Gas1 20-50% CO2/N2
Gas2 5-8% CO2/N2
Gas3 40 - 60% O2/N2
