Quantitative Real-Time PCR System Accurate 96
As a necessary choice for quantitative analysis of molecular biology, real-time PCR system has been widely used in various fields such as scientific research, clinical detection and diagnosis, quality and safety testing, and forensic applications.
· Up to 6 fluorescence detection channels allowing multiplex PCR.
· Effectively reduce multi-color crosstalk and edge effect, no ROX correction required.
· New optical scanning detection system
· Innovative scanning method and time-resolved signal separation technology
· Unique edge temperature compensation technology
· User-friendly software
| Temperature control system | |
| Sample capacity | 96 |
| Reaction volume | 10-50 μl |
| Thermal cycle technology | Peltier |
| Max. Heating/Cooling rate | 6.0° C/s |
| Heating temperature range | 4 - 100 °C |
| Temperature accuracy | ± 0.2°C |
| Temperature uniformity | ±0.2℃ @60℃ , ±0.3℃ @95℃ |
| Temperature gradient setting range | 30-100°C |
| Temperature gradient difference setting range | 1 -36°C |
| Detection system | |
| Excitation light source | 4/6 monochrome high efficiency LEDs |
| Detection device | PMT |
| Detection mode | Time-resolved signal separating technology |
| Excitation/detection wavelength range | 455-650nm/510-715nm |
| Fluorescent channels | 4/6 channels |
| Supported dye | FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/NED/TAMRA/Cy3, JUN, ROX/Texas Red, Mustang Purple, Cy5/LIZ |
| Sensitivity | Single copy gene |
| Resolution | 1.33 folds copy number difference can be distinguished in single-plex qPCR |
| Dynamic range | 10 orders of magnitude copies |
Accurate 96 is based on global vision of product design concepts and manufacturing processes. It creatively combines Fresnel lens optical signal acquisition technology, time-resolved signal separation technology and unique temperature control technology. And it reaches international advanced level in sensitivity, multi-color crosstalk, temperature uniformity and accuracy. It supports the application of all common QPCR detection modes
Simultaneous scanning of the six-channel shows that the standard deviation of the Ct value of the FAM channel is <0.07. No fluorescence signal in other channels.
Technical Innovation 1 ――Effectively reduce multi-color crosstalk and edge effect, no ROX correction required The multi-color crosstalk caused by the small sample spacing of 96 or 384-well plates has a great influence on the accuracy of the experimental results, especially in multiplex qPCR detection. The new optical signal detection system and unique time-resolved scanning can reduce non-target sample optical signal collection. Thereby high repeatability of single fluorescent channel can be ensured. Four different target genes (2 repeats) of FAM/HEX/ROX/Cy5 were simultaneously detected in one reaction tube, and the results showed that there was almost no cross-interference between the different channels.
LED light source Efficient and maintenance-free • Fresnel Lens It greatly reduces the light collection of the nontarget area. And the relative position of the detector to the block hole ensures that one optical detection channel is aligned with one target to be tested at the bottom. Different concentrations of plasmids were amplified by probe assay (concentration from left to right is 5 μg, 500 ng, 50 ng, 20 ng, 10 ng, 5 ng, 500 pg, 50 pg, 5 pg), three replicates for per concentration. The Ct values difference of the 10-fold dilution is exactly 3.3. The Ct values difference of the 2-fold dilution is exactly 1.
