The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or spectrophotometer. Endpoint, kinetic, spectrum and area-well scanning read types and PathCheck allow homogeneous and heterogeneous microplate assays to be performed in this flexible system. The FI and Abs microplate reader with built-in cuvette port allows easy conversion and optimization of very-low-throughput assays into medium-high-throughput microplate assays.
The SpectraMax M2 allows faster, more precise results and reagent savings. In addition, combined absorbance and fluorescence intensity assays can be run by issuing a single read command. Within endpoint and kinetic measurement protocols, up to four wavelength pairs can be read at a time, allowing for improved efficiency for FRET assays. Key Features: dual mode cuvette port, robotics compatible, multilabel, dual monochromators, and PathCheck.
Manufacture Specification
| Absorbance Photometric Performance | |
| Wavelength Selection | Monochromator, tunable in 1 nm increments |
| Wavelength Range | 200 to 1000 nm |
| Wavelength Bandwidth | ≤ 4.0 nm |
| Wavelength Accuracy | ± 2.0 nm |
| Wavelength Repeatability | ± 0.2 nm |
| Photometric Range | 0 to 4.0 OD |
| Photometric Resolution | 0.001 OD |
| Photometric Accuracy (Microplate) | < ±0.006 OD ±1%, to 2 OD |
| Photometric Accuracy (Cuvette) | < ±0.005 OD ±1%, to 2 OD |
| Photometric Precision | < ±0.003 OD ±1%, to 2 OD |
| Baseline Flatness | < 0.001 OD |
| Stray Light | < 0.05% @230 nm |
| Fluorescence Photometric Performance | |
| Dual Monochromators | 1 nm increment selection |
| EX Wavelength Range | 250 to 850 nm |
| EM Wavelength Range | 250 to 850 nm |
| Wavelength Bandwidth (EX, EM) | 9 nm |
| Top-Read Optimized Sensitivity | 5 pM (fluorescein) in 96 wells |
| Top-Read Guaranteed Sensitivity | 15 pM (fluorescein) in 96 wells |
| Time-Resolved Fluorescence (secondary mode) | |
| Wavelength Range | 360 to 850 nm |
| Data Collection | 50 to 1450 µsec., 200 µsec. increments |
| Guaranteed Sensitivity | 7 pM Eu-chelate |
| Luminescence (secondary mode) | |
| Wavelength Range | 360 to 850 nm |
| Guaranteed Sensitivity | 50 fM alkaline phosphatase |
| General Photometric Performance | |
| Plate Formats | 6, 12, 24, 48, 96, 384 wells |
| Light Source | Xenon flash lamp (1 joule/flash) |
| Detector | Photomultiplier tube (PMT) |
| Read Time | 96 wells: Abs 18 sec., Fl 15 sec. 384 wells: Abs 49 sec., Fl 45 sec. |
| Shaker Time | 0 to 999 seconds |
| Temperature Control | Ambient 4°C to 45°C |
| Temperature Uniformity | < 1°C at 37°C set point |
| Temperature Accuracy | ±1°C at 37°C set point |
SKU: 3337052
