6159 MP BIOTAGE PSQ-96MA DNA FRAGMENT ANALYSIS PYROSEQUENCER

From PCR product to single-stranded template ready for sequencing — up to 96 samples can be prepared in parallel using the vacuum workstation in less than 15 minutes. The workstation ensures easy handling, and the actual “hands-on time” is less than 5 minutes. Prior to Pyrosequencing, a biotinylated PCR product is generated. This biotinylated PCR product is bound to Streptavidin-coated Sepharose beads, and the beads are captured with the Vacuum Tool on the Vacuum Workstation, where they are thoroughly washed and subsequently denatured, generating single-stranded DNA suitable for Pyrosequencing. This template DNA is released into the Pyrosequencing reaction plate containing the sequencing primer, and after primer annealing, the plate is placed into the instrument. The reagents contain the enzymes, nucleotides, and substrate for the Pyrosequencing reaction; these are pipetted into the dispensing cartridge, according to the volumes provided by the software, and are also placed into the instrument for the Pyrosequencing run.