GE Biacore 4000 SPR Surface Plasmon Resonance Analyzer

System consists of:
GE Biacore 4000 SPR Surface Plasmon Resonance Analyzer
Rolling Table
Julabo Model F25 Chiller Circulator
(10) Microplate/Reservoir Tray
(10) 1000ul Buffer bottles
2000ul Buffer bottle
Waste Bottle
Windows 10 Desktop running Biacore 4000 Control software ver1.1
Windows 10 Desktop running Oracle 12c Server
Communication and power cables.

Configuration and Setup:
Sample Handling: automated sample loading and injection
Association rate (k) (proteins): 10^3 to 10^9 M^-1 s^-1, Dissociation rate (kd): 5 × 10^-5 to 1s^-1
Concentration range: 2 pM to 2 mM
Sample Volume (kinetics): 28 to 376 uL
Sample Capacity: antibodies: 10 x 96, LMW: 6 x 384

Applications: Kinetics/affinity characterization, kinetics/affinity screening, LMW interaction analysis, fragment screening, epitope mapping, immunogenicity, concentration analysis
Affinity range: fM to mM
Precision (concentration analysis): <5% CV
Molecular weight limit: > Mr 50
Baseline noise: < 0.1 RU (RMS)
Baseline drift: < 0.3 RU/min in ambient temperature range 15C to 35C
Immobilized molecule consumption: 3 to 10 ug/spot
Analysis temperature: 4C to 40C
Sample storage temperature: 4C to 40C
Data collection rate: 1 or 10 Hz
Number of flow cells: 4 x 5 spots
Unattended run time: 60 h
Data evaluation time: 120 curve sets < 6 min
GxP Support: Yes (included)
Additional Packages: antibody extension, LMW extension

Up to 4000 interactions/24 hours. [direct binding assay, 4 targets per flow cell, cycle time 5 minutes]
LMW compound screening: 1200 samples/24 hours
Antibody screening: 960 samples/24 hours
Kinetic characterization: 240 determinations/24 hours [4 targets per flow cell]

Main applications include: Fragment and LMW compound screening, Antibody and antibody fragment screening and characterization, Compound hit validation and hit to lead characterization and lead optimization of compounds.

Typical data evaluation time: Screening <1 hour, Kinetics 1 to 2 hours