Cbs Scientific Ttgek-2001-110 Cipher Ttge Electrophoresis System, 2-Place, 2 Single Cassette, 110V

Brand: C.B.S. Scientific

Features:

• C.B.S Scientific offers 2 different sized Cipher TTGE Systems which are reliable and easy-to-use
• Superior Temperature stability: Heating element and thermostat maintain excellent temperature stability (+/-0.2??) and minimize thermal stratification during DGGE
• Programmable heater/stirrer can be programmed for TTGE or DGGE applications
• Buffer Cycler eliminates need of an external peristaltic pump to recycle buffer
• This system lends itself well to the use of fluorescence based assays whose images can be captured on a fluorimeter directly through optional Borosilicate glass plate sets

CBS Scientific

Release Date: 12-03-2017

Details: Both DGGE and TTGE rely on establishing a gradient of either solvent (Urea/Form amide) or temperature in which the target fragments will undergo conformational transition (melt). This sequence dependent information will determine the theoretical melting behavior of the target fragment after PCR amplification. If the sequence is known, then primer/probe design can be made using certain software design programs. If not, the melting range can be revealed by running perpendicular DGGE/TTGE gels. The third mutation detection technique, SSCP, differentiates between normal and mutant duplex DNA by denaturing the DNA to form single stranded molecules of equal length. These molecules can re-anneal onto themselves and based on the varying degree of intrastrand base pairing, form different three-dimensional structures. These structures, differ in electrophoretic mobility and can be separated on a non-denaturing polyacrylamide gel. DGGE AND CDGE - Denaturing Gradient Gel Electrophoresis (DGGE) is a powerful genetic analysis technique that can be used for detecting single base changes and polymorphisms in genomic, cloned, and PCR amplified DNA. Two of the most valuable uses for DGGE in human and microbial genetics are in directly detecting single base changes that cause disease and in detecting polymorphisms with DNA probes for genetic-linkage analysis. In DGGE, conformational transitions of multiple nucleic acid complexes are induced by an increasing concentration of solvent (Urea/Form amide) at a constant temperature. Clinical applications of DGGE include a rapid and effective method for screening samples for genetic mutations and variants. Also, DNA fragment melting points can be determined using perpendicular DGGE (2). In contrast to DGGE, CDGE (Constant Denaturant Gel Electrophoresis) uses a single solvent percentage to induce partial melting of DNA fragments as they enter the gel. The disadvantage of CDGE is that only a single melting domain can be interrogated. TTGE -Dest