Brand: Across International
ACROSS INTERNATIONAL LLC
Details: Synonyms: agarose; protein a Purity: >98% (HPLC and SDS-PAGE) Storage: 2-8°C UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Application: Purification of mouse monoclonal antibodies from ascites fluid or cell culture supernatants. Protein A or Protein G Agarose affinity matrix can be used to purify antibodies from crude cell extracts and for the immunoprecipitation or co-immunoprecipitation of proteins. It has also been used for photoactivatable-ribonucl eoside-enhanced cross-linking immunoprecipitation (PAR-CLIP) analysis. General description: The use of recombinant Protein A in the production of Protein A agarose has the advantage that it is entirely free from Staphylococcal enterotoxins that are known to be mitogenic, and are contaminants in Protein A isolated from S. aureus . The immobilization chemistry provides high IgG-binding capacity and leak-proof stability under a wide range of pH and buffer conditions. The gel can be used over 30 times, depending on the elution condition. Protein A leakage: 18 ng Protein A/ml (ELISA) Regeneration: the gel can be used approximately 30 times Structure: recombinant Protein A (E. coli, Mr = 45,000) is covalently coupled to crosslinked 6% agarose beads: 3 mg Protein A (>98% pure, HPLC, SDS-Page)/1 ml gel Protein A, immobilized. Other Notes: For life science research only. Not for use in diagnostic procedures. Quality: Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins. Components: Preswollen gel, ready-to-use, 2 ml or 5 ml gel (settled gel volume) in buffer that contains 20% ethanol as a preservative, nonsterile. Protein A content: 3 mg/ml pre-swollen gel Specificity: Protein A is a cell wall protein, isolated from a
