Brand: Across International
ACROSS INTERNATIONAL LLC
Details: Synonyms: dissociating enzyme; neutral protease; tissue dissociating Specific Activity: ¥0.8units/mg protein (37 °C, casein as substrate, pH 7.5) UNSPSC Code: 12352200 RIDADR: NONH for all modes of transport Features and Benefits: ¢Rapid, effective, yet gentle agent that liberates cells with minimal cell damage ¢Maintains cell membrane integrity ¢Non-mammalian source - free of mycoplasma and animal virus contamination ¢Extremely stable to influences of temperature, pH, and interference by serum components ¢Easily inactivated by chelating agents or by dilution ¢Delivers higher activity and convenience Application: Dispase ® is used for the preparation of cells from a wide variety of different tissues and organs. The enzyme has proven to be a rapid, effective, yet gentle agent for the disruption of extracellular matrix of tissues for releasing single cells for cell culture (primary cell culture) or harvesting cells already in culture to transfer to new substrate (secondary culture). Furthermore, it is used to prevent unwanted clumping of cells cultured in suspension. General description: Dispase is a neutral, rapid-acting, gentle protease that separates intact epidermis from dermis. It also separates cultured intact epithelial cells from the substratum. It cleaves the basement membrane zone and leaves the epithelial cells intact. Other Notes: For life science research only. Not for use in diagnostic procedures. Preparation Note: Activator: Optimal Ca 2+ concentration is 2 mM. The enzyme preparations contain enough Ca 2+ for optimal activity. Inhibitors: EDTA, EGTA, Hg 2+ , other heavy metals. Dispase is not inhibited by serum. Working concentration: 0.6 to 2.4 U/m
