Sutter P-97 Flaming/Brown Micropipette Puller

The P-97 Flaming/Brown type micropipette puller is ideal for fabricating micropipettes, patch pipettes andmicroinjection needles. While retaining many of the features of earlier models, the P-97 offers improvements inmechanical, electronic and software design. The result is better control of the pulling process and a higher degree ofreproducibility. The P-97 combines a proven mechanical system with a sophisticated, programmable microprocessorcontroller. This programmable control of the pulling parameters allows the investigator to design application specificpipettes from a wide range of glass compositions and sizes.

A number of other features have been incorporated in the design of the P-97. Most apparent is the environmentalchamber which surrounds the heating filament. This environmental chamber is designed to minimize the effect of changinghumidity on the reproducibility of pulled pipettes. A 25% increase in power over the P-87 allows for the use of largerheating filaments, larger diameter glass and multi-barreled glass. The metal jaws that clamp the heating filament havealso been redesigned to minimize heat retention. A gas delivery mode switch provides for extended cooling for largediameter and multi-barreled glass. A spring-loaded clamping mechanism has been added for easier loading of glass. Avacuum fluorescent display has been added that allows easy viewing.

Software improvements on the P-97 include a display of the total heat-on time to assist in program development andtroubleshooting. Up to 100 programs can now be written and stored in memory, which makes the P-97 suitable for multipleusers. These programs can now be write-protected, adding security to prevent programs from being changed or alteredinadvertently. The display shows the last date and time the program was written or edited. In addition, the airpressure is included as a programmable parameter.

The P-97 contains a ramp test to overcome the difficulties of program adjustment when a new filament is installed orto help characterize a new glass and filament combination. This test allows for the rapid adjustment of heat values inestablished programs and provides estimates for establishing heat settings when using new sizes or compositions ofglass capillaries. As with all Sutter instruments, the electronics have been carefully designed to maximize noiserejection.

FEATURES

Environmental chamber Programmable air pressure Memory storage for up to 100 programs Write protection and date stampfor each program Two symmetrical pipettes with each pull Two cooling modes: time and delay Preprogrammed sampleprograms for intracellular and patch pipettes. Special programming on request Ramp test - self test for establishingprogram heat settings when a new filament or glass is introduced Vacuum fluorescent display Constant current powersupply for filament and pull solenoid Looping pull cycle for fabrication of patch type micropipettes Self-contained airsupply with filtration system and humidity control Consistent and reliable electrodes with tip diameters less than 0.1mControl over the time and pressure at which the air is delivered Optimized velocity sensing circuit for maximizedsensitivity and reproducibility Quality control, SEM photograph of a tip pulled with each puller; criterion is tipmeasurement less than 0.1m and typically is ~0.06m

TECHNICAL SPECIFICATIONS

Dimensions
21in x 16in x 12in | 53cm x 40.6cm x 30cm

Weight
50 lbs | 23 kg

Electrical
115/230 volts | 50/60 Hertz power line